Design of an Uniform Magnetic Field Exposure System for Experiments of Biological Effects of Magnetic Fields on Culture Cells
○Yoshiko Nakano, Keiko Hosokawa*，Hisao Yamaguchi*，Ki Ho Park, Toshitaka Ikehara*，
Mituo Kitamura*，Yohsuke Kinouchi, Kazuo Yoshizaki*，Hiroshi Miyamoto**
Faculty of Engineering,The University
of Tokushima. School of medicine ,The University of Tokushima. Tokushima Bunri University
I Introduction
Our goal is to made in vitro experiment which produce ELF(extremely low frequency) magnetic fields.Until now, many results were reported, however effect of magnetic field on living body has not definited yet.In this study, we designed of an Uniform Magnetic Field Exposure System .
II Previous Experimental Apparatus
Magnetic fields produced by the coils were sinusoidal(60 Hz) and their rms values were from 1.5 to 3 mT(error of 50%). The culuture dishes were placed in two special incubators to keep the temperature of the cultures constant (37±0.2℃).
fig.1 Previous Experimental Apparatus
Previous experimental apparatus have a margin of error 50%.We have to improve it in quality. So, we designed new model as below.
III calculation of magnetic flux density
Fig.2; We propose new system insted of previous one. This system have characteristic that we set up petri dish in coil. Bz shows magnetic flux densitiy of vertical direction.
fig.2 Model
fig.3; We calculated magnetic flux densitiy of new model. Magnetic flux densitiy of Center of coil is 3.0[mT], maximum value is about 3.5[mT]. A margin of error was 11.8 %.
fig.4; In order to get more high-performance, we improved new model. Modified designing used double coil for regulating function.We calcuated magnetic flux densitiy of modified designing model.
fig.3 result of calculation
fig.4 Modified designing
fig.5; We measured magnetic flux densitiy of new model(60[Hz],1.38[A],first coil;29.5[A],second coil;5.0[V]).We obtained uniform Magnetic Field(error 4.2%).
fig.5 Actual measurement
fig.6 Experimental Apparatus
IV Prospect
We have designed an Uniform Magnetic Field Exposure System.Actual measurement reflected Result obtained from the computer simulation.We show culture system and temperature variation(fig.7).
![[fig.1 Previous Experimental Apparatus ]](figure.files/sochi.jpg)
![[fig.2 Model ]](figure.files/jikai.jpg)
![[fig.3 result of calculation ]](figure.files/keisan.jpg)
![[fig.4 Modified designing ]](figure.files/new.jpg)
![[fig.5 Actual measurement ]](figure.files/jissoku.jpg)
![[fig.6 Experimental Apparatus ]](figure.files/syasin2.jpg)
![[fig.7 Temperature variation ]](figure.files/ondo.jpg)