Session 1 Chair: Yukyo Takada (Tohoku University)



An extremely low magnetic field exposure in dental pulp stem cells


M. Hata, M. Omi, R. Fukuzawa, N. Kojima, Y. Nakamura, S. Ozawa, and Y. Tanaka


Departments of Removable Prosthodontics, School of Dentistry, Aichi-Gakuin University




 Dental pulp stem cells (DPSCs) are located in dental pulp and DPSCs can differentiate into several type of cells including dentin and osteoblasts. In this study, we have investigated the response of an extremely low magnetic field in DPSCs. DPSCs were isolated and cultured from 6-week-old male Sprague-Dawley rats by previously described. The identification of DPSCs were analyzed by a fluorescence activated cell sorter (FACS) analysis. Cells were differentiated adipocytes or osteoblasts using differentiation medium. To examine the response of an extremely low magnetic field in DPSCs, cells were exposed by magnetic field which was 0.4T and 0.17Hz for 6 hours. The proliferation and osteogenic differentiation were analyzed with MTT assay, ALP staining and osteocalcin immunostaining. This stimulation significantly increased the growth of DPSCs by 1.5 times and osteocalcin positive cells. In conclusion, an extremely low magnetic field exposure may be thought to promote application of DPSCs for bone regeneration.